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41 Questions on Cell and Molecular Biology with Solution | BIOL 2104, Exams of Cellular and Molecular Biology

Material Type: Exam; Professor: Finkielstein; Class: Cell and Molecular Biology; Subject: Biological Sciences; University: Virginia Polytechnic Institute And State University; Term: Spring 2007;

Typology: Exams

Pre 2010

Uploaded on 02/10/2007

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Download 41 Questions on Cell and Molecular Biology with Solution | BIOL 2104 and more Exams Cellular and Molecular Biology in PDF only on Docsity! Name: ID# 1 Exam #1 February 14th 1- The primary limiting factor for resolution in a light microscope is the: a- quality of the lenses b- numerical apertures c- index of refraction d- angle of illumination into the objective lens e- wavelength of light 2- Which instrument gives the most โ€œ3-dimentional-likeโ€ image (greatest Z-axis of focus): a- light microscope b- transmission electron microscope c- scanning electron microscope d- immunofluorescence microscope e- a single-lens microscope 3- In its optical design, the transmission electron microscope is most like a(n): a- scanning electron microscope b- single-lens microscope c- confocal microscope d- compound light microscope (brigtfield) 4- Which instrument is best used to view the structure of virus? a- single-lens microscope b- confocal microscope c- scanning electron microscope d- transmission microscope e- brightfield microscope 5- Living, unstained cells and organisms can be observed best using: a- fluorescent microscopy b- transmission electron microscopy c- phase contrast microscopy d- scanning electron microscopy 7- Transmission electron microscopy is best for high magnification viewing of: a- internal structure of fixed cells b- internal structure of live, motile cells c- surface structure of fixed cells d- surface membranes of live, motile cells 8- The color of the light that is emitted by a fluorophore is different from the color of the light that has been absorbed because: a- the light energy emitted has a shorter wavelength than the energy absorbed b- the light energy emitted has a longer wavelength than the energy absorbed c- the light energy emitted and absorbed has similar energies Name: ID# 2 d- the color of the light is independent of the energy emitted by the fluorophore but it depends on the wavelength of the energy absorbed 9- What would be the most suitable subject for study in yeast? a- photosynthesis b- mouse mating behavior c- cell cycle regulation d- cell motility in response to external stimulus e- organogenesis (organ development) 10- Arabidopsis is an auxotrophic organism (require one or more specific substances for growth and metabolize that the parental organism was able to synthesize on its own) a- True b- False 11- The genome of Arabidopsis has very little repetitive DNA. a- True b- False 12- Arabidopsis information processing genes are homologous to genes found in yeast and mammals. a- True b- False 13- Which of the following organisms has the smallest genome? a- Arabidopsis thaliana b- Gallus gallus c- Homo sapiens d- Mus musculus e- Saccharomyces cerevisiae 14- Name the vertebrate model organism best suited for studies of genetics and developmental biology: a- Danio rario b- Drosophila melanogaster c- Escherichia coli d- Saccharomyces cerevisiae e- Xenopus laevis 15- The ___________ is unique among model organisms in that it can be grown and genetically manipulated with the speed and ease of a microorganism while offering the features of a real animal. It is the best free-living soil characterized multicellular animal at the level of genomics, genetics, embryology, and its genome is fully sequenced. a- Escherichia coli b- Drosophila melanogaster c- Caenorhabditis elegans d- Schizosaccharomyces pombe Name: ID# 5 Lagging strand D. an RNA polymerase DNA ligase E. DNA sequence where replication begins helicase F. is synthesized in pieces Okazaki fragments G. Catalyzes linkage of one deoxyribonucleotide to another DNA polymerase III H. Joins Okasaki fragments together primase I. Relieves tension as helix is unwound during replicain Leading strand J. Molecular molds for fashioning new strands of DNA a- I,H,E,J,B,C,F,G,D,A b- E,J,I,F,H,B,C,G,D,A c- E,G,D,J,H,B,A,C,I,F d- B,G,C,E,J,I,H,F,D,A e- A,I,H,B,E,J,F,D,G,C 27- During DNA synthesis, the leading strand is synthesized ______ and the lagging strand is synthesized _______ a- 3โ€™ to 5โ€™; 5โ€™ to 3โ€™ b- 3โ€™ to 5โ€™; 3โ€™ to 5โ€™ c- 5โ€™ to 3โ€™; 3โ€™ to 5โ€™ d- 5โ€™ to 3โ€™; 5โ€™ to 3โ€™ 28- Which of the following subunits of bacterial RNA polymerase recognize specific DNA sequences? a- alpha only b- sigma only c- beta and betaโ€™ d- alpha, beta and betaโ€™ e- none of the above 29- Enhancers are: a- proteins located adjacent to promoters b- distant sites where regulatory proteins bind c- expediters of RNA polymerase capture d- proteins that bind with repressors, deactivating them e- a bacterial form of promoters 30- Bacterial RNA polymerase core binds to a __________ to form a holoenzyme that recognize promoters a- sigma factor b- TFII c- -35 sequence d- TATA box Name: ID# 6 31- Eukaryotic mRNA transcripts are protected from modification by a- 5โ€™ caps b- 5โ€™ poly-A caps c- 3โ€™ caps d- 5โ€™-3โ€™ poly tails 32- The Shine-Dalgarno sequence: a- is a sequence in 23S rRNA that basepairs with the 5โ€™UTR of a eukaryotic mRNA b- is a sequence in a bacterial mRNA that is on the 3โ€™ side of the AUG start codon c- is a sequence in the 16S rRNA that interacts with the CAU anticodon of the initiator tRNA d- is a sequence in a bacterial mRNA that basepairs with the 16S rRNA e- None of the above 33- Formation of peptide bonds during translation is catalyzed by: a- RNA in the small ribosomal subunit b- RNA in the large ribosomal subunit c- Proteins in the large ribosomal subunit d- elongation factor-G e- water 34- In the charging of tRNAs: a- Most tRNA synthetases read the anticodons on the tRNAs to determine what amino should be used b- The amino acid is first activated by putting AMP onto its carboxyl end c- In the second step, a covalent bond is formed between the 5โ€™ end of the tRNA and the carboxyl group of the amino acid, AMP is released d- Amino acid-AMP is released and rebound by the tRNA synthetase, the cycle of binding and release is coupled to ATP hydrolysis e- None of the above 35- As polypeptides are formed at the ribosome, elongation continues until __________ is exposed a- a release factor b- an intron c- a nonsense codon d- an exon e- polypeptidase Name: ID# 7 BONUS QUESTIONS (PLEASE ANSWER THE QUESTIONS IN THESE SHEET) 1- The x-ray diffraction photography of Rosalind Franklin and Maurice was critical evidence of DNA โ€จ a- indicating that DNA has a double helix structure โ€จb- showing equal number of purines and pyrimidines โ€จc- showing the bases of DNA were held together by hydrogen bonds โ€จd- revealing the structure of the deoxyribose sugar โ€จe- of the location of each adenine, guanine, cytosine, and thymine. 2- The direction of replication of DNA is a- 5' to 3' b- 3' to 5' c- 5' to 5' d- 3' to 3' e- variable 3- Which protein(s) are not NOT part of the pre-replication complex? a- ORC complex b- pol alpha/primase c- MCM complex d- Cdc6 e- Cdt1 4- Label the indicated proteins or DNA molecules (with the indicated letters) in the drawing. a- leading-strand template b- newly synthesized DNA strand c- sliding clamp d- DNA polymerase on leading strand e- parental DNA helix f- new okazaki fragment g- DNA helicase h- DNA primase i- single-strand DNA-binding protein j- new Okazaki fragment k- DNA polymerase on lagging strand l- RNA primer m- DNA polymerase on lagging strand n- location of a newly sinthezised Okasaki fraqment
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