Biochemistry Exam: Second Exam for 694:301 Course - Prof. F. Deis, Exams of Biochemistry

Download Biochemistry Exam: Second Exam for 694:301 Course - Prof. F. Deis and more Exams Biochemistry in PDF only on Docsity! Biochemistry 694:301 Name_________________________ Second Exam, Dr. Deis Last 5 digits of SSN ___________________ Monday Aug. 5, 2002 Row Letter ____ Seat Number ____ This exam consists of two parts. Part I is multiple choice. Each of these 25 questions is worth two points. Answer the Part I questions on this sheet, below. Answer the Part II questions on the question pages. Please use BLOCK CAPITAL letters like this --- A, B, C, D, E. Not lowercase! 1. ______ 10. ______ 18. ______ 2. ______ 11. ______ 19. ______ 3. ______ 12. ______ 20. ______ 4. ______ 13. ______ 21. ______ 5. ______ 14. ______ 22. ______ 6. ______ 15. ______ 23. ______ 7. ______ 16. ______ 24. ______ 8. ______ 17. ______ 25. ______ 9. ______ GRADE: Part I Total _______ Part II: II-1 _______ II-2 _______ II-3 _______ II-4 _______ II-5 _______ Part II Total _______ Total, I & II _______ 694:301 Second Exam, page 2 Name ________________________ 1. Which of the following statements about the sodium channel is incorrect? A. it is sensitive to voltage B. it becomes inactivated spontaneously C. it consists of seven hydrophobic transmembrane segments D. it is much more permeable to Na+ than to K+ E. none of the above 2. Acetylcholinesterase has A. five subunits B. a negatively charged area around the active site C. a reactive cysteine at the active site D. an ATP Binding Cassette E. none of the above 3. For a simple (uncharged) osmotic process, the standard free energy will be A. zero D. negative B. positive E. you can't tell without specifics C. one 4. Which of the following water soluble vitamins forms part of Coenzyme A? A. folate D. thiamine B. pyridoxine E. pantothenate C. riboflavin 5. If PEP + ADP --> ATP + Pyruvate has a ∆Go' = –7.5 kcal /mol, using your knowledge of ATP hydrolysis, what is the standard free energy change for the hydrolysis of PEP --> Pyruvate + Pi ? A. –14.8 D. +10.2 B. –10.2 E. + 14.8 C. –0.2 6. The Citric Acid Cycle is part of which of "stage" of catabolism? A. I C. III B. II D. not part of catabolism 7. Protein Kinase A causes phosphorylation of the target sequence RRGSI in such enzymes as Glycogen Synthase and Phosphorylase Kinase. PKA is normally bound to an inhibitor which has the altered sequence: A. ARGSI D. RRGAI B. RAGSI E. RRGSA C. RRASI 8. One of the second messengers in Protein Kinase C activation is strongly hydrophobic. It is often known by its abbreviation A. PIP2 D. SRO B. CPU E. DAG C. IP3 694:301 Second Exam, page 5 Name ________________________ 1. Give specific answers to each question: a1. Where is Acetylcholinesterase located? (2) a2. Why is it such a fast-acting enzyme? (2) a3. What happens to an animal exposed to an inhibitor of ACHase. (2) b. Compare and contrast the Calcium pump (Ca2+ ATPase) and the Multidrug Resistance Protein. Which is a P-type ATPase? What does each do in the cell? Be as specific as you can. (4) 694:301 Second Exam, page 6 Name ________________________ 2.* a. What cofactors are represented below? (6) b. Show how Glucose-6-Phosphate can be converted to Ribulose-5-P by the oxidative branch of the Pentose Phosphate Pathway. Draw all reactants and products, indicate all cofactors, and name all enzymes. Roughly 1/2 point per fact. Hint, start with G6P drawn in the Haworth ring form. (4) 694:301 Second Exam, page 7 Name ________________________ 3. a. Show how Fructose-1,6-BP would be converted into Phosphoenolpyruvate by the enzymes of Glycolysis. Draw all reactants and products, name all enzymes, and indicate all cofactors. 1/2 point per fact. Note, you may simply write out the entire pathway if that is easier for you. (8) b. Galactose is converted into Glucose by a 3-epimerase discussed in class. Show the reaction, being careful to represent the correct reactant and product. You may use "sticks" where appropriate but do show the epimerization. (2)