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Exam 1 - Flashcards

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Class:BIOL 402 - Fundmntals of Microbiology Lab
Subject:Biology
University:University of Kansas
Term:Fall 2014
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Which agent seemed to be most effective at removing microbes from the fingerprints? (1-1) The iodine and the chlorhexidine should be at the top, they are used prior to surgeries
What other variables besides the cleansing agent might be responsible for any differences noted between fingers? (1-1) Variables such as the surface chosen for sampling degree of contamination, how vigorously it was sampled, individual's skin texture, the duration and intensity of finger scrubbing, the amount of agent used, drying method (air v towel) and the pressure applied by the finger to the agar, among others, can affect results.
Did you or any other students notice some microbes persisting more than others after washing?What factor(s) might account for this? (1-1) IF YES, then cell wall composition, type of organism (bacterium, fungus...) and density of the microbial population could affect hardiness of certain microbes
RESEARCH: Triclosan (1-1) Bactericidal (not endospores). Affects fatty-acids biosynthesis and membrane transport at lower concentrations and membrane integrity at higher concentrations.

Antiprotozoal, minor fungicide, antiviral (primarily enveloped viruses)
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RESEARCH: Isopropyl/ethyl alcohol (1-1) Bactericidal (Gr+and Gr-, not endospores); protein denaturation


Fungicidal and virucidal (enveloped viruses)
RESEARCH: Benzalkonium chloride 1, 2 (1-1) Bacteristatic, damages cell membranes and denatures proteins, including critical enzymes


Virucidal (enveloped viruses)
RESEARCH: Povidone iodine scrub 1,2,3 (1-1) Bactericidal (Gr+ and some Gr-; some endospores) damages protein and nucleic acid structure and interfered with their synthesis also damages cell membranes

Fungicidal and virucidal (enveloped viruses)
RESEARCH:Chlorhexidine gluconate1,3 (1-1) Bactericidal (Gr+ and Gr-; not endospores) and bacteriostatic (depending on duration of application and concentration); affects cell membrane integrity (by binding to proteins) and causes precipitation of cytoplasmic componentsFungicidal and virucidal (enveloped and non-enveloped viruses)
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Hardest medium to transfer from? (1-3) Slant? --gain the use of the pinky finger to achieve holding the cap and in one hand while transfering
IF you got growth on sterile NA and NB slant rubes, why? (1-3) Possible contamination include not heating the loop to orange-hot, not holding the open tubes on an angle, and/or placing the cap on the table surface during the process.
Did you notice a difference in density (turbidity) of growth in NB tubes inoculated from NB and NA slants? Possible reasons. (1-3) Generally, more cells are transferred from growth on a solid medium than from a broth culture. Therefore, broth cultures made from growth on a solid medium will show greater turbidity than those inoculated from a broth culture.
Did you notice a difference in density of growth on NA slants inoculated from NA slants and NB? (1-3) Generally there will be denser growth on the slant inoculated from the NA slant, because more cells are transferred from solid medium than broth.
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Using a pencil, draw a quadrant streak (1-4) KNOW HOW TO DRAW IT
If one doesn't get isolation with a quadrant streak, what happened? (1-4)
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 Which agent seemed to be most effective at removing microbes from the fingerprints? (1-1)The iodine and the chlorhexidine should be at the top, they are used prior to surgeries
 What other variables besides the cleansing agent might be responsible for any differences noted between fingers? (1-1)Variables such as the surface chosen for sampling degree of contamination, how vigorously it was sampled, individual's skin texture, the duration and intensity of finger scrubbing, the amount of agent used, drying method (air v towel) and the pressure applied by the finger to the agar, among others, can affect results.
 Did you or any other students notice some microbes persisting more than others after washing?What factor(s) might account for this? (1-1)IF YES, then cell wall composition, type of organism (bacterium, fungus...) and density of the microbial population could affect hardiness of certain microbes
 RESEARCH: Triclosan (1-1)Bactericidal (not endospores). Affects fatty-acids biosynthesis and membrane transport at lower concentrations and membrane integrity at higher concentrations.

Antiprotozoal, minor fungicide, antiviral (primarily enveloped viruses)
 RESEARCH: Isopropyl/ethyl alcohol (1-1)Bactericidal (Gr+and Gr-, not endospores); protein denaturation


Fungicidal and virucidal (enveloped viruses)
 RESEARCH: Benzalkonium chloride 1, 2 (1-1)Bacteristatic, damages cell membranes and denatures proteins, including critical enzymes


Virucidal (enveloped viruses)
 RESEARCH: Povidone iodine scrub 1,2,3 (1-1)Bactericidal (Gr+ and some Gr-; some endospores) damages protein and nucleic acid structure and interfered with their synthesis also damages cell membranes

Fungicidal and virucidal (enveloped viruses)
 RESEARCH:Chlorhexidine gluconate1,3 (1-1)Bactericidal (Gr+ and Gr-; not endospores) and bacteriostatic (depending on duration of application and concentration); affects cell membrane integrity (by binding to proteins) and causes precipitation of cytoplasmic componentsFungicidal and virucidal (enveloped and non-enveloped viruses)
 Hardest medium to transfer from? (1-3)Slant? --gain the use of the pinky finger to achieve holding the cap and in one hand while transfering
 IF you got growth on sterile NA and NB slant rubes, why? (1-3)Possible contamination include not heating the loop to orange-hot, not holding the open tubes on an angle, and/or placing the cap on the table surface during the process.
 Did you notice a difference in density (turbidity) of growth in NB tubes inoculated from NB and NA slants? Possible reasons. (1-3)Generally, more cells are transferred from growth on a solid medium than from a broth culture. Therefore, broth cultures made from growth on a solid medium will show greater turbidity than those inoculated from a broth culture.
 Did you notice a difference in density of growth on NA slants inoculated from NA slants and NB? (1-3)Generally there will be denser growth on the slant inoculated from the NA slant, because more cells are transferred from solid medium than broth.
 Using a pencil, draw a quadrant streak (1-4)KNOW HOW TO DRAW IT
 If one doesn't get isolation with a quadrant streak, what happened? (1-4)
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